Transport in Plants Question Answers: NCERT Class 11 Biology

Diffusion is the passive movement of substances from a region of higher concentration to a region of lower concentration. Diffusion of substances plays an important role in cellular transport in plants. Rate of diffusion is affected by concentration gradient, membrane permeability, temperature, and pressure. Diffusion takes place as long as there is a difference between the concentrations of a substance across a barrier. However, diffusion stops, when the concentrations of the substance on either side of the barrier become equal. The permeability of a membrane affects the rate of diffusion. Diffusion rate increases as membrane permeability increases. Changes in temperature and pressure values also affect the diffusion of substances. Pressure plays an important role in the diffusion of gases as gases diffuse from a region of higher partial pressure to a region of lower partial pressure.

Root pressure is the positive pressure that develops in the roots of plants by the active absorption of nutrients from the soil. When the nutrients are actively absorbed by root hairs, water (along with minerals) increases the pressure in the xylem. This pressure pushes the water up to small heights. Root pressure can be observed experimentally by cutting the stem of a well-watered plant on a humid day. When the stem is cut, the solution oozes from the cut end.

Root pressure is also linked to the phenomenon of guttation, i.e., the loss of water in the form of liquid droplets from the vein endings of certain herbaceous plants.

Root pressure is only able to transport water up to small heights. However, it helps in re-establishing the continuous chains of water molecules in the xylem. Transpirational pull maintains the flow of water molecules from the roots to the shoots.

In tall trees, water rises with the help of the transpirational pull generated by transpiration or loss of water from the stomatal pores of leaves. This is called the cohesion-tension model of water transport. During daytime, the water lost through transpiration (by the leaves to the surroundings) causes the guard cells and other epidermal cells to become flaccid. They in turn take water from the xylem. This creates a negative pressure or tension in the xylem vessels, from the surfaces of the leaves to the tips of the roots, through the stem. As a result, the water present in the xylem is pulled as a single column from the stem. The cohesion and adhesion forces of the water molecules and the cell walls of the xylem vessels prevent the water column from splitting.

In plants, transpiration is driven by several environmental and physiological factors. The external factors affecting transpiration are wind, speed, light, humidity, and temperature. The plant factors affecting transpiration are canopy structure, number and distribution of stomata, water status of plants, and number of open stomata. Although transpiration causes water loss, the transpirational pull helps water rise in the stems of plants. This helps in the absorption and transport of minerals from the soil to the various plant parts. Transpiration has a cooling effect on plants. It helps maintain plant shape and structure by keeping the cells turgid. Transpiration also provides water for photosynthesis.

Transpirational pull is responsible for the ascent of water in the xylem. This ascent of water is dependent on the following physical factors:

• Cohesion – Mutual attraction between water molecules

• Surface tension – Responsible for the greater attraction between water molecules in liquid phase than in gaseous phase

• Adhesion – Attraction of water molecules to polar surfaces

• Capillarity –Ability of water to rise in thin tubes.

These physical properties of water allow it to move against gravity in the xylem.

In plants, nutrients are absorbed through the active and passive transports. The endodermal cells of the roots containing suberin allow only selected minerals to pass through them. The transport proteins present in the membranes of these cells act as check points for the various solutes reaching the xylem.

During the growth of a plant, its leaves act as the source of food as they carry out photosynthesis. The phloem conducts the food from the source to the sink (the part of the plant requiring or storing food). During spring, this process is reversed as the food stored in the sink is mobilised toward the growing buds of the plant, through the phloem. Thus, the movement of food in the phloem is bidirectional (i.e., upward and downward).

The transport of water in the xylem takes place only from the roots to the leaves. Therefore, the movement of water and nutrients in the xylem is unidirectional.

According to the pressure flow hypothesis, food is prepared in the plant leaves in the form of glucose. Before moving into the source cells present in the phloem, the prepared food is converted into sucrose. Water moves from the xylem vessels into the adjacent phloem, thereby increasing the hydrostatic pressure in the phloem. Consequently, the sucrose moves through the sieve cells of the phloem. The sucrose already present in the sink region is converted into starch or cellulose, thereby reducing the hydrostatic pressure in the sink cells. Hence, the pressure difference created between the source and the sink cells allows sugars to be translocated from the former to the latter. This starch or cellulose is finally removed from the sink cells through active transport.

The tiny pores present on the surfaces of leaves, called stomata, help in the exchange of gases. Each stoma consists of bean-shaped or dumbbell-shaped guard cells. The epidermal cells surrounding the guard cells are modified to form subsidiary cells. The opening and closing of the guard cells is caused by a change in their turgidity. The inner walls of the guard cells are thick and elastic, while the outer walls are thin. The numerous microfibrils present in the guard cells facilitate the opening and closing of the guard cells.

At the time of the opening of the stomata, the turgidity of the guard cells increases. As a result, the outer walls bulge and the inner walls become crescent-shaped. The stomatal opening is facilitated by the radial arrangement of the microfibrils.

At the time of the closing of the stomata, the guard cells lose their turgidity, the outer and inner walls retain their original shapes, and the microfibrils get arranged longitudinally.

Porins are types of proteins which form pores of large sizes in the outer membranes of plastids such as chloroplast, mitochondria and the membranes in bacteria. They help in facilitating the passive transport of small-sized protein molecules.

In plant cells, active transport occurs against the concentration gradient, i.e., from a region of lower concentration to a region of higher concentration. The process of active transport involves specific protein pumps. The protein pumps are made up of specific proteins called trans-membrane proteins. These pumps first make a complex with the substance to be transported across the membrane, using the energy derived from ATP. The substance finally gets liberated into the cytoplasm as a result of the dissociation of the protein–substance complex.

Water potential quantifies the tendency of water to move from one part to the other during various cellular processes. It is denoted by the Greek letter Psi or Ψ. The water potential of pure water is always taken as zero at standard temperature and pressure.

It can be explained in terms of the kinetic energy possessed by water molecules. When water is in liquid form, the movement of its molecules is rapid and constant. Pure water has the highest concentration of water molecules. Therefore, it has the highest water potential. When some solute is dissolved in water, the water potential of pure water decreases.

(a) Diffusion and osmosis

(b) Transpiration and evaporation

(c) Osmotic pressure and osmotic potential

(d) Imbibition and diffusion

(e) Apoplast and symplast pathways of movement of water in plants

(f) Guttation and transpiration

Water potential quantifies the tendency of water to move from one part to the other during various cellular processes such as diffusion, osmosis, etc. It is denoted by the Greek letter Psi or Ψ and is expressed in Pascals (Pa). The water potential of pure water is always taken as zero at standard temperature and pressure.

Water potential (Ψw) is expressed as the sum of solute potential (Ψs) and pressure potential (Ψp).

Ψw = Ψs + Ψp

When some solute is dissolved in water, the water potential of pure water decreases. This is termed as solute potential (Ψs), which is always negative. For a solution at atmospheric pressure, Ψw = Ψs.

The water potential of pure water or a solution increases on the application of pressure values more than atmospheric pressure. It is termed as pressure potential. It is denoted by Ψp and has a positive value, although a negative pressure potential is present in the xylem. This pressure potential plays a major role in the ascent of water through the stem.

The water potential of pure water or a solution increases on the application of pressure values more than atmospheric pressure. For example: when water diffuses into a plant cell, it causes pressure to build up against the cell wall. This makes the cell wall turgid. This pressure is termed as pressure potential and has a positive value.

(a) Plasmolysis can be defined as the shrinkage of the cytoplasm of a plant cell, away from its cell wall and toward the centre. It occurs because of the movement of water from the intracellular space to the outer-cellular space. This happens when the plant cell is placed in a hypertonic solution (i.e., a solution having more solute concentration than the cell cytoplasm). This causes the water to move out of the cell and toward the solution. The cytoplasm of the cell shrinks and the cell is said to be plasmolysed. This process can be observed in an onion peel kept in a highly concentrated salt solution.

(b) When a plant cell is placed in a hypertonic solution or a solution having higher water potential, the water diffuses into the cell (i.e., movement is observed from higher to lower water pressure region). The entry of water in the plant cell exerts pressure on the rigid cell wall. This is called turgor pressure. As a result of its rigid cell wall, the plant cell does not burst.

Mycorrhiza is a symbiotic association of fungi with the root systems of some plants. The fungal hyphae either form a dense network around the young roots or they penetrate the cells of the roots. The large surface area of the fungal hyphae is helpful in increasing the absorption of water and minerals from the soil. In return, they get sugar and nitrogenous compounds from the host plants. The mycorrhizal association is obligate in some plants. For example, Pinus seeds do not germinate and establish in the absence of mycorrhizal.

Yes, plants need to adjust the types of solutes that reach the xylem. The transport proteins of epidermal cell helps to adjust the solute movement. Older dying leaves export their minerals like phosphorus, Sulphur, nitrogen, potassium etc. to younger leaves. Similarly before leaf falling in deciduous plants minerals are exported to the other parts of plants. Some elements like calcium are not remobilized.

Plants can be grow under limited water supply without compromising on metabolic activities when a plant gets limited supply of water then it brings a lot of changes in physiology to prevent loss of water.

Some examples are:

In xerophytes by preventing transpiration, water loss is prevented.

Thus plants have the capability to manage with limited supply of water.

No, it is not possible, Ascent of sap is not possible without cohesion and adhesion of water molecules. There are several other factors like root pressure, capillary action, adhesion, cohesion and transpiration that are involved in ascent of sap in plants. Root pressure can only move water up to the base of the stem. Capillary action is effective only in herbs because it can raise water only up to few centimeters. Transpiration works only when there is water column underneath to be pulled. The continuous water flow in xylem vessels for ascent of sap is regulated by cohesive and adhesive forces of water molecules. If these forces are not present then it will lead to the breakage of water column or the sap movement upwards. 

The tissue which is conducting the water up the stem is xylem. Hence, this experiment can demonstrate that xylem is conducting water up the stem.

1. This phenomenon is called plasmolysis.
2. If the filament is replaced in distilled water, the water flows in the shrunken protoplasm which results in swelling up of protoplasm or gets deplasmolysed.

Sugar crystals do not dissolve easily in cold water but do so in hot water because in hot water number of striking water molecules is higher than that water which is ice- cold. The kinetic energy of water reduces with reduction in temperature. Thus the dissolution of solid crystals of sugar does not occur in ice- cold water.

Salt solution is a hypertonic solution thus causes exosmosis in plants. 1 cup salt in 2 cup of water, fairly well dissolved when sprayed on weed plants start killing them.

Xylem sap: The xylem sap mainly contains inorganic compounds most of Sulphur and potassium and most of the nitrogen in the form of organic compound. Water and concentration is in dilute form. Thus it is mildly acidic.

Phloem sap: Both organic and inorganic compounds are present. As organic compounds help in translocation of food and inorganic compounds help in exchange of materials between xylem and phloem.

Tube B shows higher water rise than A because the lumen of tube B is smaller than tube A. thus tube B creates more capillary effect than tube A. So the level of rising of water is more in that as well. 

Aquaporins are kind of membrane proteins. These proteins help in facilitating the transport of water between the cells. Thus, presence of aquaporins would increase the rate of osmosis.

a. ABA is called stress hormone because during stress conditions it induced changes like closing of stomata during scarcity of water to prevent further loss of water. In this way ABA overcome stress condition.

b. This hormone gets released in leaves from the mesophyll cells of plant.

Some plants capable of survival under flooding. There is deprivation of oxygen in the plant roots causing an anaerobic condition. Thus aeration is affected.

Diffusion is the process of movement of substances from a region of their lower concentration. This is a passive process. Energy is not required. Facilitates movement of water and gases.

Translocation is the movement of materials from leaves to other tissues throughout the plant. This is an active transport. Facilitates movement of organic compounds.

Diffusion is different from facilitated diffusion as diffusion occurs through phospholipid layer and facilitated diffusion occurs through membrane proteins.

Mass flow hypothesis is also known as the press flow hypothesis. It was proposed by Munch in 1930 and it is accepted as most scientific. It states that when there is a high concentration of sugar present in the source then a diffusion gradient gets created between the sugar source and the sugar shrink where the sugar is stored. As the pressure is involved and movement of substance takes place in bulk thus this is called Mass Flow hypothesis or Pressure Flow hypothesis.

1. These types of guard cells are found in dicots.
2. Stomata in figure: (i) show higher water content because it is turgid.
3. Potassium plays an important role in the opening and closing stomata.

Uniport: When a single substance moves in a single direction across the cell membrane.

Antiport: When two substances move in the opposite direction across a cell membrane.

Symport: When two substances move in the same direction across a cell membrane.

No, they do not need energy directly.

1. Plasma lemma: Selectively permeable
2. Tonoplast: Selectively permeable
3. Parchment membrane: Semi- permeable
4. Egg membrane: Semi- permeable

Halophytes are the plants which adapted to live in saline water. Salt solution has the higher concentration. So their cell cytoplasm is hypertonic causing water from the surrounding cells to enter the cell cytoplasm. These cell store large amount of salt (sodium and potassium salt) in their vacuoles. This helps the plants in maintaining the pre- cell pressure which is much higher than atmospheric pressure.

The radiolabeled carbon in carbon dioxide supplied to potato plants in an experiment was seen in the tuber eventuoally. When the potato plant carried out photosynthesis using the CO₂ which is radiolabeled, it forms glucose (C₆H₁₂O₆) and oxygen. Glucose molecule has the radiolabeled carbon present in it. Auto radiography is used to measure this which detects the radioactive carbon and trace the components along with the movement in the plant body.

The upward movement of the water through xylem vessels leaves a transpiration pull which does not break due to adhesion along with adhesion and surface tension, transpiration pull, pull up the water column in plants. Thus, during ascent of sap in plants a lot of factors work together.

1. The set up demonstrate the effect of wind speed on rate of transpiration.
2. The level of water decreases if the blower is placed close to the setup.
3. Yes, if the phenyl mercuric acetate is sprayed on the leaves then it will reduce the transpiration rates. As the result mercury level would go down.

In a girdled plant, when water is supplied to the leaves remain green for some time because leaves synthesizes their food by photosynthesis using H₂O and CO₂ in presence of sunlight.

Various types of transport mechanisms are needed to fulfil the mineral requirements of a plant. They are not fulfilled by diffusion alone because plants also required mineral nutrients and charged ions like potassium, sodium etc. which cannot be transported through the simple diffusion because the cell membrane which is selectively permeable as it does not allow charged particles pass through it.