Restriction enzymes that are used in the | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

Question:

Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?

Answer:

The restriction enzyme cuts double helical segments which contains particular nucleotide sequence which is cut down by the restriction endonuclease enzyme at specific recognition sequence. If the restriction enzyme doesn’t cut the DNA at specific position, it will not produce any sticky ends (cleavage does not falls on symmetrical axis, as in results restriction fragments will give sticky ends) and recombination will also not occur. Instead of giving sticky ends it will produce blunt ends when it cut down from the random position.

cut ends


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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 4: Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut....