A mixture of fragmented DNA was electrop | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

Question:

A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethidium bromide, no DNA bands were observed. What could be the reason?

Answer:

A mixture of DNA fragments was electrophoresed in agarose gel and after staining with the ethidium bromide, no bands were appeared. There could be many reasons for that and these are following:-

May be the staining concentration is not enough to stain the DNA fragments so that the bands appears.
Isolation process can be degraded and no enzymes worked on break down of cell envelope.
By improper cutting or it could be occur because of endonuclease when cuts on random position or when exonuclease and endonuclease cuts at the same time; or can be loaded in the DNA sample on the agarose gel.

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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 8: A mixture of fragmented DNA was electrophoresed in an agarose gel. After staining the gel with ethid....

A mixture of fragmented DNA was electrop | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

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