Discuss the significance of heavy isotop | Class 12 Biology Chapter Molecular Basis of Inheritance, Molecular Basis of Inheritance NCERT Solutions

Question:

Discuss the significance of heavy isotope of nitrogen in the Meselson and Stahl’s experiment.

Answer:

They performed experiments on E.coli to prove that DNA replication is semi- conservative. They first grew the bacteria in a medium containing ¹⁵NH₄Cl for many generations.

Then, transferred the cells into a medium with normal ¹⁴NH₄Cl (with the light nitrogen) and collects the samples at different definite time intervals as the cells multiplied. The extracted DNAs were then centrifuged and measured to get their appropriate densities.

The DNA extracted from the culture after one generation of transfer from then ¹⁵N (heavy nitrogen) medium to ¹⁴N- this shows an intermediate hybrid density, i.e., both heavy and light nitrogen, which proved the semi- conservative nature of DNA.

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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 3: Discuss the significance of heavy isotope of nitrogen in the Meselson and Stahl’s experiment.....

Discuss the significance of heavy isotop | Class 12 Biology Chapter Molecular Basis of Inheritance, Molecular Basis of Inheritance NCERT Solutions

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