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A plasmid DNA and a linear DNA (both are of the same size) have one si...

A plasmid DNA and a linear DNA (both are | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

Question:

A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.

Answer:

Plasmid DNA shows one band after cutting by restriction endonuclease because it is a circular shaped DNA. When it is cut down then it will open up and show linear DNA fragment. And when linear DNA is cut down with specific restriction endonuclease enzyme it will divide into two fragments after cleaving and hence show two bands when we put this on the agarose gel electrophoresis.

CUT ENDS

Size of the DNA fragments depends on cleavage site or its position that is from when it cuts the DNA molecule.

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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 5: A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonucle....

A plasmid DNA and a linear DNA (both are | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

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