Name the five key tools for accomplishin | Class 12 Biology Chapter Biotechnology and its Application, Biotechnology and its Application NCERT Solutions

Question:

Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.

Answer:

Recombinant DNA technology is a technique which enables the DNA to be identified, isolated and then recombined by which new features can be introduced into the organism. Specific tools that are used to accomplish the recombinant DNA technology with their functions are as follows:

Restriction endonuclease: The restriction enzyme cuts double helical segments which contains particular nucleotide sequence which is cut down by the restriction endonuclease enzyme at specific recognition sequence.
Vector: The term refers to the DNA molecule which acts as transporting vehicle which carries foreign DNA into the host cell for the cloning (method that involves the replication of single DNA molecule to generate a large population of cells and every cell contains identical DNA molecules.) and expression.
DNA Ligase: DNA ligase enzyme is used to join the DNA molecules by making the Phosphodiester bond between the DNA at the two different molecules end.
DNA polymerase: It is an enzyme which synthesizes new polynucleotides complementary to an existing DNA or RNA template both are included in this category.
Host cell or DNA delivery system: the cell which takes up recombinant DNA and changes itself to the transformant.

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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 10: Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the ....

Name the five key tools for accomplishin | Class 12 Biology Chapter Biotechnology and its Application, Biotechnology and its Application NCERT Solutions

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