Restriction enzymes should not have more | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

Question:

Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.

Answer:

The restriction enzyme cuts double helical segments which contains particular nucleotide sequence which is cut down by the restriction endonuclease enzyme at specific recognition sequence of four to eight base pairs in length, is known as restriction or recognition site. Cloning vectors contain various features which allows foreign DNA to be inserted into the vectors that may be known as multiple cloning site or polylinker site which contains various unique restriction sites which are cleaved by restriction enzymes and then targeted gene is ligated into the vectors. If it will have more than one restriction site it will form various fragments by which no specific result will form.


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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 4: Restriction enzymes should not have more than one site of action in the cloning site of a vector. Co....