A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect the experiment?
Selectable marker encodes a product which allows the artificial selection and growth of transformed or transfected cells. The cloning vector needs the presence of selectable marker used to identify and eliminate the non- transformant cell. Antibiotic resistance gene are mostly used as selectable markers and as well as antibiotics. A host cell strain is chosen which will react to the particular antibiotics like ampicillin, tetracycline, kanamycin etc. which are been used in transformation process.
So, if there will be no selectable marker, no identification of transformed cell that contains DNA molecule will occur.
Note*:- Ampicillin:- it inhibits the bacterial cell wall synthesis.
Tetracycline:- It inhibits the binding of aminoacyl tRNA to the 30S ribosomal subunit.
Kanamycin:- it inactivates the translation process.
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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 7: A plasmid without a selectable marker was chosen as vector for cloning a gene. How does this affect ....
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