How does one visualise DNA on an agarose | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

Question:

How does one visualise DNA on an agarose gel?

Answer:

Gel electrophoresis technique is used to separate the DNA fragments, in which matrix, agarose gel is used. DNA fragments moves towards the anode according to their molecular size through this gel, and then the smaller fragments get separated from the larger fragments. And, these separated DNA fragments can be only observed after staining with the solution of ethidium bromide. Bright orange colour bands will appear only under the UV- light. Ethidium bromide is the most commonly used fluorescent dyes which is used to intercalate between bases of nucleic acids and their fragments. It is used because of its short staining time.

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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 6: How does one visualise DNA on an agarose gel?....

How does one visualise DNA on an agarose | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

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