Welcome to the NCERT Solutions for Class 12 Biology - Chapter Biotechnology Principles and Process. This page offers a step-by-step solution to the specific question from Exercise 0, Question 7: . With detailed answers and explanations for each chapter, students can strengthen their understanding and prepare confidently for exams. Ideal for CBSE and other board students, this resource will simplify your study experience.
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
PCR chain reaction is carried out by the thermostability of the polymerase. PCR contains a series of cycles of three successive steps:- Denaturation, primer annealing and DNA synthesis; These three steps constitutes one complete cycle which is carried out repetitively just by the change in temperature.
Denaturation is the first step of the PCR reaction, in which the strands of duplex DNA are separated by heat at about 93- 95◦C. If this separation will not occur then primers will also not be able to anneal to complementary segments on each strand and extension will also not take place. Hence no DNA synthesis will occur and will have negative effect on the process.
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