While doing a PCR, ‘denaturation&r | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

Question:

While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?

Answer:

PCR chain reaction is carried out by the thermostability of the polymerase. PCR contains a series of cycles of three successive steps:- Denaturation, primer annealing and DNA synthesis; These three steps constitutes one complete cycle which is carried out repetitively just by the change in temperature.

Denaturation is the first step of the PCR reaction, in which the strands of duplex DNA are separated by heat at about 93- 95◦C. If this separation will not occur then primers will also not be able to anneal to complementary segments on each strand and extension will also not take place. Hence no DNA synthesis will occur and will have negative effect on the process.

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Welcome to the NCERT Solutions for Class 12 Biology - Chapter . This page offers a step-by-step solution to the specific question from Excercise 0 , Question 7: While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process....

While doing a PCR, ‘denaturation&r | Class 12 Biology Chapter Biotechnology Principles and Process, Biotechnology Principles and Process NCERT Solutions

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